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Dr. Anand’s Review invitation for EMP_2016_108 (Experimental and Molecular Pathology)

Dr. Anand’s Review invitation for EMP_2016_108 (Experimental and Molecular Pathology)

Ref: EMP_2016_108
Title: Down-regulation of epithelial membrane protein-1 is associated with HNSCC cell proliferation
Journal: Experimental and Molecular Pathology
Corresponding Author: Zezhang Tao
Co-authors: Yang Xiling, Wang Lei, Hu Zhangwei, Xu Yong, Zheng Anyuan, Li Fen, Xiao Bokui, Chen Chen

Dear Dr Srivastava,

I would like to invite you to review the above-referenced manuscript. To maintain our journal’s high standards we need the best reviewers, and given your expertise in this area I would greatly appreciate your contribution.

I kindly ask you to give this review invitation the same consideration that you would want one of your own manuscripts to receive. Please find the abstract of the manuscript at the end of this email.

If you are willing to review this manuscript, please click on the link below:

Please also note that authors have been invited to convert their supplementary material into a Data in Brief article (a data description article). You may notice this change alongside the revised manuscript. You do not need to review this, but may need to look at the files in order to confirm that any supporting information you requested is present there.

I look forward to receiving your response.

Kind regards,

Julius Cruse
Experimental and Molecular Pathology


To investigate epithelial membrane protein-1 (EMP1) gene function in Head and Neck Squamous Cell Carcinoma (HNSCC) tumorigenesis and development. We first used GEO dataset to analyze the EMP1 gene expression in HNSCC. Second, we investigated the EMP1 protein expression in tissue microassay to analyze the relationship between EMP1 protein expression and TNM stage. Third, the co-expression genes data of EMP1 were extracted from Cancer Cell Line Encyclopedia database(CCLE database) , and their correlation were analyzed in The Cancer Genome Atlas HNSCC database(TCGA HNSCC database). Next, the GO (Gene Ontology Analysis) and KEGG Pathway enrichment analysis were carried out. Finally, we overexpressed the EMP1 in HNSCC cell line Cal-27, protein expression were analyzed by Western blot respectively as well as cell viability and migration were evaluated by Cell Counting Kit-8 (CCK-8) and transwell chamber assay. Analysis of GEO dataset concluded that EMP1 gene is down-regulated in HNSCC tumor tissues compared with normal tissues (P<0.05). There was no statistically significant difference of EMP1 gene expression between different T-stage and N-stage tumor tissues (P>0.05). The results of TMA showed that EMP1 protein expression in tumor was lower than that in normal tissue (P<0.05). Expression of EMP1 protein was not correlated with TNM stage of HNSCC. In addition, The Cal-27 overexpressed EMP1 significantly decreased cell viability and the invasion significantly reduced (P<0.05). After EMP1 overexpression ITGA3 and EPHA2 genes were significantly up-regulated, while ANXA1 gene was significantly down-regulated. Our data demonstrated that EMP1 gene down-regulation involved in the HNSCC tumorigenesis and development. It may serve as a novel biomarker for prognostic evaluation of patients with HNSCC.

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