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Title: Down-regulation of epithelial membrane protein-1 is associated with HNSCC cell proliferation
Journal: Experimental and Molecular Pathology
Corresponding Author: Zezhang Tao
Co-authors: Yang Xiling, Wang Lei, Hu Zhangwei, Xu Yong, Zheng Anyuan, Li Fen, Xiao Bokui, Chen Chen
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Experimental and Molecular Pathology
To investigate epithelial membrane protein-1 (EMP1) gene function in Head and Neck Squamous Cell Carcinoma (HNSCC) tumorigenesis and development. We first used GEO dataset to analyze the EMP1 gene expression in HNSCC. Second, we investigated the EMP1 protein expression in tissue microassay to analyze the relationship between EMP1 protein expression and TNM stage. Third, the co-expression genes data of EMP1 were extracted from Cancer Cell Line Encyclopedia database(CCLE database) , and their correlation were analyzed in The Cancer Genome Atlas HNSCC database(TCGA HNSCC database). Next, the GO (Gene Ontology Analysis) and KEGG Pathway enrichment analysis were carried out. Finally, we overexpressed the EMP1 in HNSCC cell line Cal-27, protein expression were analyzed by Western blot respectively as well as cell viability and migration were evaluated by Cell Counting Kit-8 (CCK-8) and transwell chamber assay. Analysis of GEO dataset concluded that EMP1 gene is down-regulated in HNSCC tumor tissues compared with normal tissues (P<0.05). There was no statistically significant difference of EMP1 gene expression between different T-stage and N-stage tumor tissues (P>0.05). The results of TMA showed that EMP1 protein expression in tumor was lower than that in normal tissue (P<0.05). Expression of EMP1 protein was not correlated with TNM stage of HNSCC. In addition, The Cal-27 overexpressed EMP1 significantly decreased cell viability and the invasion significantly reduced (P<0.05). After EMP1 overexpression ITGA3 and EPHA2 genes were significantly up-regulated, while ANXA1 gene was significantly down-regulated. Our data demonstrated that EMP1 gene down-regulation involved in the HNSCC tumorigenesis and development. It may serve as a novel biomarker for prognostic evaluation of patients with HNSCC.
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Dear Dr. Anand S Srivastava,
Hope you are doing well.
At first, I sincerely apologize if am disturbing you in your hectic schedule.
We are glad to let you know that Journal of Stem Cell Research & Therapeutics (2475-5540) have successfully released the former issues in 2016.
Moreover we are running with Volume 2 issue 2 and we are in need of one article to accomplish this issue. In fact we have gone through your eminent articles in online and my desire is to fulfill the issue with your article.
Hence I humbly request you to submit your manuscript (Review/Mini review/Case Report) at the earliest. Your prompt submission will help me to release the issue in time and will impact my ranking too.
Hope you will understand us and look forward to receive your manuscript as early as possible.
Await your reply.
Hi Dr. Anand S. Srivastava,
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